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Immuno-regulatory effects on murine macrophages by low dose naltrexone (LDN)

Title
Immuno-regulatory effects on murine macrophages by low dose naltrexone (LDN)
Publication Type
Journal Article
Research Type
Lab (in-vitro)
Reported as
Date
January 28, 2016
Authors
Sheng-Nan Guo, Yan Li, Xiao-Nan Wang, Feng-Ping Shan
Institution
China Medical University
Link
Abstract

OBJECTIVE: The effect of naltrexone on the function of murine peritoneal macrophages in vitro was studied in order to illuminate its immune activity further.

METHODS: Peritoneal macrophages were divided in three groups: RPMI1640 blank control group, LPS positive control group and NTX treated group. Various phenotypic and functional indices were tested by MTS, flow cytometry technology, phagocytosis experiment and ELISA.

RESULTS: Compared with the RPMI1640 group, LDN exhibited paradoxical properties; the expression of CD64 on the surface increased while the expression of CD206 decreased in the NTX group; the expression of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) were increased.

CONCLUSION: The results of the experiment proved that LDN could influence macrophage polarization, regulate inflammatory mediator production and affect the phagocytic function of peritoneal macrophages.